TAR – RNA aptamer loop - loop interaction by X - ray crystallography , UV spectroscopy and surface plasmon resonance
نویسندگان
چکیده
CNRS-Université Bordeaux 1-ENITAB, UMR 5248 CBMN, Institut Européen de Chimie et Biologie, Pessac, F-33607, France. Synchrotron SOLEIL, L’Orme des Merisiers, Saint-Aubin, B.P. 48, 91192 Gif-sur-Yvette Cedex, France. INSERM U869, Institut Européen de Chimie et Biologie, Pessac, F-33607, France Université de Bordeaux, Bordeaux, F-33076, France. 5 Present address: CNRS-Université Louis Pasteur, UMR 7104 IGBMC, Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1 rue Laurent Fries BP 10142, F-67404 Illkirch Cedex France. 6 Present address: CESAMO, Université de Bordeaux, 351 cours de la Libération, Talence, F33405 cedex, France.
منابع مشابه
Exploring TAR–RNA aptamer loop–loop interaction by X-ray crystallography, UV spectroscopy and surface plasmon resonance
In HIV-1, trans-activation of transcription of the viral genome is regulated by an imperfect hairpin, the trans-activating responsive (TAR) RNA element, located at the 5' untranslated end of all viral transcripts. TAR acts as a binding site for viral and cellular proteins. In an attempt to identify RNA ligands that would interfere with the virus life-cycle by interacting with TAR, an in vitro s...
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Riboswitch RNAs fold into complex tertiary structures upon binding to their cognate ligand. Ligand recognition is accomplished by key residues in the binding pocket. In addition, it often crucially depends on the stability of peripheral structural elements. The ligand-bound complex of the guanine-sensing riboswitch from Bacillus subtilis, for example, is stabilized by extensive interactions bet...
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The trans-activating responsive (TAR) RNA element located in the 5' untranslated region of the HIV-1 genome is a 57-nt imperfect stem-loop essential for the viral replication. TAR regulates transcription by interacting with both viral and cellular proteins. RNA hairpin aptamers specific for TAR were previously identified by in vitro selection [Ducongé,F. and Toulmé,J.J. (1999) In vitro selectio...
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تاریخ انتشار 2008